Endocrine Abstracts

Objective: To investigate the relationship between expression of TLR2, TLR4, and TLR9 in PBMCs and type 2 diabetes and their significance in pathogenesis of diabetes.

Methods: Expression of TLR2, TLR4, and TLR9 in PMBCs was measured by flow cytometry in 112 subjects with coronary heart disease (CHD, n=49), type 2 diabetes plus coronary heart disease (DM+CHD, n=34), type 2 diabetes (DM, n=4) and healthy controls (CONTROL, n=25) were analyzed. The results were calculated by MFI and MPP respectively.

Results: There was no significant difference of expression of TLR2, TLR4, and TLR9 in PMBCs among study groups. The positive rate or MFI of TLR2 in monocyte was significantly higher than that in lymphocyte. There was no significant difference of expression of TLR4 and TLR9 between monocyte and lymphocyte respectively.

Conclusions: There is no significant relationship between the expression of TLR2, TLR4, TLR9 in PMBCs and diabetes. TLR2 is mainly expressed in human monocytes. TLR4 and TLR9 are expressed in both human monocytes and lymphocytes.

Keywords: Type 2; Diabetes; Coronary heart disease; Toll-like receptors; flow cytometry