ECE2013 Poster Presentations Cardiovascular Endocrinology & Lipid Metabolism (41 abstracts)
CNR1 polymorphisms and metabolic disorders in woman with PCOS-preliminary report
Andrzej Milewicz 1 , Łukasz Łaczmański 1 , Agnieszka Lenarcik 1 , Justyna Kuliczkowska 1 , Anna Trzmiel-Bira 1 , Barbara Stachowska 1 , Diana Jędrzejuk 1 , Ewa Sadowska 1 , Katarzyna Kolackov 1 , Maurycy Pawlak 1 , Anna Arkowska 1 , Urszula Dorobisz 2 , Lidia Hirmle 3 & Felicja Lwow 4
1Department of Endocrinology, Diabetology and Isotope Therapy, Wroclaw Medical University, Wroclaw, Poland; 2Department of Radiology, Wroclaw Medical University, Wroclaw, Poland; 3Department of Gynecology, Wroclaw Medical University, Wroclaw, Poland; 4Department of Health Promotion, Faculty of Physiotherapy, University School of Physical Education, Wroclaw, Poland.
The endocannabinoid system has been suspected to contribute to the association of visceral fat accumulation with metabolic diseases. Insulin resistance play important role in etiology in PCOS. The aim of our study was developed the role of CNR1 polymorphisms (rs806368, rs12720071, rs1049353, 806381, rs10485170, rs6454674, rs2023239) in metabolic disorders in woman with PCOS. 88 woman (20–35 years old) with PCOS (recognized using Rotterdam criteria) and 77 woman (20–35 years old) as homogenous controls group were study. Lipids profile and carbohydrate metabolism in serum using standard methods were study. Genomic DNA were isolated using standard method. To amplify the genetic material of the PCR technique was used. To polymorphisms identification minisequencing was used. Reaction products were separated on Genetic Analyser ABI 3100. For statistic analysis ANOVA test was used.
In polymorphism rs12720071 eight woman with PCOS present genotype G/G in contrast to control we can’t find such a genotype. These genotype in polymorphism rs1049353 in PCOS group present a higher WHR vs other genotype in contrast to control group. Also other polymorphism rs 10485170 in genotype A/G show significantly lower LDL cholesterol levels in controls but not in woman with PCOS. In polymorphism rs2023239 genotype C/C was connected with elevated levels of free androgens index in PCOS patients.
Our preliminary results can suggest the potential role of CNR1 polymorphisms PCOS etiology what need further study.
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Endocrine Abstracts
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