SFEBES2013 Poster Presentations Pituitary (71 abstracts)
The effect of mTOR blockers on japanese quail ovarian granulosa cell functions
Attila Kadasi 1 , Nora Maruniakova 1 , Adriana Kolesarova 1 , Andrej Balazi 2 , Emilia Hanusova 3 , Jan Kotwica 4 , Antonello Mai 5 & Alexander V Sirotkin 2
1Slovak University of Agriculture, Nitra, Slovakia; 2Institute for Genetics and Reproduction of Farm Animals, Animal Production Research Centre, Nitra-Luzianky, Slovakia; 3Institute of Animals Breedingand Product Quality, Animal Production Research Centre Nitra, Nitra-Luzianky, Slovakia; 4Institute of Animal Reproduction and Food Research, Olsztyn- Kortowo, Poland; 5Instituto Pasteur, Fondazione Cenci Bolognezzi, Universita degli Studi di Roma, La Sapienza, Roma, Italy.
The aim of our study was to elucidated the role of mTOR-dependent intracellular signalling pathway in control of ovarian functions. For this purpose, we have examine the effect of three mTOR inhibitors (resveratrol, curcumin and synthetic mTOR blocker MC 2183 – Mai et al. 20051, at the doses 0, 1, 10, 100 μg/ml) on apoptosis and steroidogenesis by cultured Japanese quail ovarian granulosa cells. The release of steroid hormones (progesterone and testosterone) and accumulation of bax (marker of apoptosis) was analysed by RIA and immunocytocxhemistry respectively.
It was observed, that resveratrol adition decreased progesterone release (at 1 and 10 μg/ml but not at 100 μg/ml) and stimulated testosterone release (at 10 and 100 μg/ml but not at 1 μg/ml), as well as increased the percentage of apoptotic (bax-positive) cells at dose-dependent manner at all doses (1, 10 and 100 μg/ml) added. Curcumin treatment diminished progesterone release (at 10 and 100 μg/ml but not at 1 μg/ml), activated both testosterone release (at 10 and 100 μg/ml but not at 1 μg/ml) and apoptosis at all doses (at 1, 10 and 100 μg/ml). MC 2183 addition significantly down-regulated progesterone secretion (at 1 and 100 μg/ml but not at 10 μg/ml), did not affected testosterone release at any dose (at 1, 10 and 100 μg/ml) and increased accumulation of bax (at 10 and 100 μg/ml but not at 1 μg/ml).
These observations suggest the involvement of mTOR-dependent intracellular pathway in control of ovarian steroidogenesis and apoptosis. It can be involved in promotion of progestogen, inhibition of androgen and suppression of apoptosis in avian ovarian cells.
References:: 1. Mai et al. 2005 Design, syntesis, and biological evaluation of sirtinol analouges as class III histone/protein deacetylase (sirtuin) inhibitors. Int. J. Med. Chem. 2005 48 7789–7795.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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