Endocrine Abstracts

Introduction: Mechanism underlying glucose-mediated development and progression of diabetic complication are incompletely understood. Atrial natriuretic peptide (ANP) is a hormone released by atrial myocytes in response to acute and chronic extracellular volume expansion. Moderate hyperglycaemia (>400 mg/dl) in diabetic rats is associated with an elevation in circulating ANP levels and in GFR (glomerular filtration rate), (1) and play a role in the development of microalbuminuria (2).

Elevated plasma ANP concentrations have been reported in human diabetic subjects with poor glycaemic control although these findings have not been confirmed by other studies (3).

Materials and methods: We included 21 diabetic patients, with ages (X±DS) 61.3±12.6 years and 8.4±9 years of diabetes progression. They were 2 type 1 diabetes and 19 were type 2.

We analyzed levels of ANP levels by RIA.

Results: -There was no change in the levels of ANP in hyper-or normoglycaemia (90.52±47.48 pg/ml vs. 86.98±37.74 pg/ml). P=0.56.

-The average levels of glucose in hyperglycaemia were 294.85±75.19 mg/dl and 134±34.16 mg/dl in normoglycaemia.

Conclusion: -Hyperglycaemia did no induce changes in the ANF levels in our study.

-This result could be influenced by the higher prevalence of type 2 diabetics in our group with higher basal levels of ANF.

-Furthermore, the level of hyperglycemia was not too high in our work and this can be a stimulus course shorter than in other published studies with more marked hyperglycaemia.

Bibliography: 1-Francoise V. Ortola, Barbara J. Ballermann, Sharon Anderson, Ramon E. Mendez, and Barry M. Brenner. Elevated Plasma Atrial Natriuretic Peptide Levels in Diabetic Rats. J. Clin. Invest. Volume 80, September 1987, 670–674.

2-K Mc Kenna; D. Smith; Tormey and C. j. Thompson. Acute hyperglycemia causes elevation in plasma atrial natriuretic peptide concentration in type 1 diabetes mellitus. Diabetic Medicine 2000; 17, 512–517.

3-Trevisan R, Fioretto P; Semplicini A, Opocher G, Mantero F. Role of insulin and ANF in sodium retention in insulin treated IDDM patients during isotonic volume expansion. Diabetes 1990; 49: 289–298.

Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.

Funding: This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.