Endocrine Abstracts

Accurate and sensitive detection of the biomarkers is important both for preclinical research fields such as biochemistry, biomedicine, and clinical diagnostics. Immunosensors are the miniaturized analytical devices that combine high specificity of immunological reaction with sensitivity of detection techniques.

In this study, we developed a new competitive immunosensor with employing antibody (Ab) labeled AuNP (Ab-AuNP) and mobile crystalline material-41 (MCM-41)-polyvinyl alcohol (PVA) mesoporous nanocomposite modified screen-printed carbon electrode (SPCE) surface to detect the urine albumin. Field emission scanning electron microscopy (FE-SEM) of modified electrode showed a suitable and stable attachment between HSA antigen – mAb and AuNP. Cyclic voltammetric (CV) method demonstrated that modification process was well performed.

Differential pulse voltammetry (DPV) was employed for quantitative determination of antigen in biological samples. The electrochemical measurements performed with other proteins mixed with samples demonstrated a high specificity and selectivity for this biosensor in detecting the HSA. In optimal conditions, this immunosensor could detect HSA in a high linear range (0.5–200 μg/ml) with a low detection limit of 1 ng/ml. The proposed method showed acceptable reproducibility, stability, and reliability and could also be applied to detect the other antigens.

Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.

Funding: This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.