Endocrine Abstracts

Type 1 diabetes mellitus (T1DM) not only is congenital disease that known as insulin-dependent diabetes, often occurs in children and adolescents. Recent advances in quantitative proteomics including fluorescence two-dimensional differential gel electrophoresis (2D-DIGE) and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) have offered opportunities to discover plasma proteins as biomarkers for tracking the progression and for understanding the molecular mechanisms of diabetes. Proteomic analysis of the plasma proteome in T1DM and healthy donors indicated that 41 proteins representing 36 unique proteins are T1DM associated biomarkers including heme-associated protein, hemopexin. Further investigation of glucose mediated hemopexin induction was determined in vitro. Our data indicated that hemopexin can be induced in numerous cell lines (ARPE19, chang’s liver cells, HT29 and Hela) by increasing the glucose concentration in the medium. Interestingly, the glucose-induced hemopexin overexpression can be reduced by ROS scanvenger such as glutathion implying hemopexin expression is linked to glucose-induced oxidative stress. To sum up, the current work has identified numerous T1DM specific biomarkers by proteomic strategy. In addition, to our knowledge, this is the first report showing the expression of hemopexin can be modulated by glucose concentration which is mediated by ROS.

Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.

Funding: This work was supported, however funding details unavailable.