Endocrine Abstracts

Objective: The aim of the study was to evaluate distribution of circulating adiponectin (AdipoQ) isoforms at pregnant women with gestational diabetes mellitus (GDM) and comparison analysis of isoform distribution changes 12 months after delivery.

Design: The study was conducted on 64 pregnant women who underwent routine pre-natal tests for GDM between 24 and 28 week of gestation and 30 of them 12 months after delivery. The study group included 36 women diagnosed with GDM based on the results of 75 g OGTT and the control group of 28 healthy pregnant women with a normal screening test and no risk factors for GDM.

Methods: Quantitative determination of total AdipoQ and its isoforms in serum was performed with the multimeric adiponectin ELISA (Alpco Diagnostics, Salem, NH, USA). The assays were run according to the manufacturer recommendations.

Results: Total AdipoQ concentration in women with GDM was significantly lower than in women without GDM. Among adiponectin isoforms significantly lower concentrations of HMW multimers and LMW trimers in women with GDM were found in comparison to the control group during pregnancy, while the concentration of MMW hexamers did not differ between the groups. After delivery a significant increase in total AdipoQ as well as in the concentration of HMW and LMW was found in women with the history of GDM. While searching for correlations between different forms of AdipoQ we found that HMW and MMW negatively correlated with BMI before and during gestation as well as after delivery.

Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.

Funding: This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.