ICEECE2012 Poster Presentations Cardiovascular Endocrinology and Lipid Metabolism (74 abstracts)
Effect of triiodothyronine and angiotensin-II on expression of (pro)renin receptor in the human erythroid cell line, YN-1
H. Nishiyama 1 , K. Kaneko 1 , K. Ohba 1 , T. Yoshioka , T. Shimizu 1 , T. Hirose 2 , K. Totsune 3 & K. Takahashi 1
1Tohoku University Graduate School of Medicine, Sendai, Japan; 2Tohoku University Graduate School of Pharmaceutical Sciences and Medicine, Sendai, Japan; 3Tohoku Fukushi University, Sendai, Japan.
The renin–angiotensin system is known to enhance erythropoiesis. (Pro)renin receptor ((P)RR), a specific receptor for renin and prorenin, has recently been identified. We have recently shown expression of (P)RR in a human erythroid cell line, YN-1. It is known that triiodothyronine (T3) and angiotensin-II (A-II) stimulate proliferation of erythroid cells. The aim of the present study is to clarify effects of T3 and A-II on expression of (P)RR in the human erythroid cell line, YN-1. YN-1 cells were maintained in Iscove’s modified Dulbecco’s medium containing 10% fetal bovine serum. To study effects of T3 and A-II on (P)RR expression, the cells were cultured in the medium containing T3 (10 and 100 nM) or A-II (10 and 100 nM) for 24 h. Expression of (P)RR mRNA and protein was examined by real time RT-PCR and western blot analysis respectively. The antiserum against (P)RR was raised in a rabbit by injecting the peptide fragment of human (P)RR corresponding to 224–237 a.a. (Hirose et al. 2009). Real time RT-PCR showed that T3 increased expression levels of (P)RR mRNA significantly in a concentration dependent manner. T3 caused a 1.6-fold increase at 100 nM compared with control. Western blot analysis showed increased expression of (P)RR protein by T3 although this increase was not statistically significant. By contrast, A-II had no significant effects on (P)RR mRNA and protein levels in YN-1 cells. In this study, we have found that T3 stimulates expression of (P)RR in YN-1 cells, suggesting a possibility that (P)RR is related to erythropoiesis induced by T3.
Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.
Funding: This work was supported, however funding details unavailable.
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