ICEECE2012 Poster Presentations Thyroid cancer (108 abstracts)
Remarkable enhance in CYP24A1 expression in human thyroid cancer tissue
B Balla , J Kósa , B Tóbiás , C Halászlaki , I Takács , H Horváth , G Speer , J Horányi , Z Nagy , B Járay , E Székely & P Lakatos
Semmelweis University, Budapest, Hungary.
Background: It is well established that 1,25-dihydroxyvitamin D3 (1,25-D3) inhibits cell growth and induces apoptosis in numerous tumors. Increased expression of 1,25-D3 inactivating enzyme, (24-hydroxylase - CYP24A1), has been observed in numerous thyroid cancer cell lines after calcitriol treatment in vitro. We examined the expression of CYP24A1 as well as the activating 1-α-hydroxylase (CYP27B1) gene in human thyroid cancer tissue.
Methods: The gene expression analyses of thyroid carcinoma samples were carried out by Taqman probe-based quantitative real-time RT-PCR. Total RNA was isolated from each sample with Roche High Pure Total RNA Isolation kit.
Results: CYP24A1 mRNA expression was markedly increased in all but one papillary cancers compared to that of normal thyroid tissue, reaching sometimes 300-fold elevation. No significant alteration was seen in CYP27B1 gene activity between neoplastic and normal tissues.
Conclusions: If the observed increase in CYP24A1 expression proves to be true on a larger samples size of human thyroid cancers, the use of higher doses of vitamin D3 and/or the development of CYP24A1 inhibitors or 24-hydroxylase-resistant vitamin D analogues could open a new approach to the effective treatment of thyroid cancers.
Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.
Funding: This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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