1NHIC Ilsan Hospital, Goyang-si, Republic of Korea; 2College of Medicine, Yonsei University, Seoul, Republic of Korea.
Objective: Cathepsin L can control adipogenesis and relate to acute coronary syndrome. However, it is not clear whether cathepsin L can be affected by saturated fatty acid and decrease by curcumin. We examined the hypothesis that palmitate upregulates cathepsin L expression in adipose tissue and curcumin can be block that effect.
Methods: 3T3-L1 cells were fully differentiated for 8 to 10 days and treated with palmitate, LPS, IL6, TNF-α, IL6 neutralizing Ab, curcumin. Six-week-old C3H/HeN with normal TLR4 and C3H/HeJ with TLR4 mutation got LPS injection ip (1 mg/kg) or were fed with high fat diet for 13 weeks and sacrifice to harvest epididymal fat.
Results: Real-time PCR revealed that cathepsin L expression was upregulated by palmitate. But there was no additional effect when we use palmitate-treated RAW264.7 cells’ media instead of direct treatment to 3T3-L1 cells. Cathepsin L was upregulated after treatment of TNF-α or IL6 like palmitate. IL6 neutralizing Ab and curcumin attenuated this effect of palmitate. Cathepsin L increased in both kinds of mice fed high fat diet compared with chow diet. But we cannot find similar pattern at mice which got LPS ip injection.
Conclusions: We concluded that cathepsin L expression in fat are upregulated by palmitate via inflammatory cytokines, not TLR4, thereby might have a critical role in developing acute coronary syndrome of metabolic syndrome and there are no additional effects of infiltrating macrophages. In addition, curcumin can be a candidate of drug which can amilorate the metabolic syndrome.
Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.
Funding: This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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