ICEECE2012 Poster Presentations Thyroid (non-cancer) (188 abstracts)
Laminin and poly-laminin, a polymeric form of laminin assembled at acidic pH, differentially modulate PCCL3 thyroid cell behavior
C. Palmero , M. Barroso , E. Souza , C. Merlmestein , C. Takiya , D. Carvalho , L. Miranda-Alves & L. Nasciutti
Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
The extracellular matrix protein laminin forms polymers both in vivo and in vitro. Acidification of pH leads to the formation of an artificial polymer with biomimetical properties, named poly-laminin (polyLM). Follicular cells in the thyroid are in close contact with laminin, but their response to this important extracellular signal is still poorly understood. Thyroid PCCL3 epithelial cells cultivated either on glass, regular laminin (LM) or laminin previously polymerized in acidic pH (polyLM) showed distinct cell morphologies and proliferative rates, as well as differences in the organization of their actin cytoskeleton. While on polyLM cells displayed a typical epithelial morphology and presented actin fibers radially organized, on LM they spread irregularly on the substrate, lost cell contacts and produced thick actin fibers, which expanded to the whole cytoplasm. From a functional standpoint, both substrates of laminin induced a slight decrease in the expression of the sodium iodine symporter (NIS), but a significant decrease in iodine uptake was detected when cells are cultivated on laminin in comparison with cells cultivated on glass. In this study we demonstrated that PCCL3 cells can discriminate between LM and polyLM and that they respond to the latter by better preserving their phenotype as in the thyroid tissue.
Declaration of interest: The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project.
Funding: This work was supported, however funding details unavailable.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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