ECE2010 Poster Presentations Growth factors (15 abstracts)
Decreased expression of apoptotic genes in skeletal muscles, but not in hearts, of GH receptor knockout (GHRKO) mice
Adam Gesing 1, , Michal Masternak 1 , Feiya Wang 1 , Andrzej Lewinski 3 , Malgorzata Karbownik-Lewinska 2 & Andrzej Bartke 1
1Department of Internal Medicine, Geriatrics Research, Southern Illinois University School of Medicine, Springfield, Illinois, USA; 2Department of Oncological Endocrinology, Chair of Endocrinology and Metabolic Diseases, Medical University of Lodz, Lodz, Poland; 3Department and Chair of Endocrinology and Metabolic Diseases, Medical University of Lodz, Lodz, Poland.
GH receptor knockout (GHRKO) mice were produced by a targeted disruption of the GH receptor (Ghr) gene. These long-lived mice, which are GH resistant, are characterized by a greatly reduced plasma levels of IGF1 and insulin and have enhanced insulin sensitivity (these effects resemble the characteristics of animals subjected to caloric restriction (CR), which is well known experimental model to delay aging and increase lifespan).
Apoptosis, or programmed cell death, is a normal component of the development and health of multicellular organisms and is a process in which cells play an active role in their own death (which is why apoptosis is often referred to as cell suicide).
The aim of the study was to examine the expression of genes related to apoptosis (using real-time PCR) in the homogenates of skeletal muscles and hearts collected from female GHRKO mice subjected to CR (40%; 6 months), starting at 2 months of age.
Results: Expression of the examined apoptotic genes (caspase 3, caspase 9, bax, Smac/DIABLO) is decreased in skeletal muscles of GHRKO mice as compared to normal controls (P<0.03, P<0.007, P<0.04, P<0.04, respectively); no statistical differences were observed for the genes in question in hearts of GHRKO mice (in comparison to normals). CR increased caspase 3 expression in homogenates of hearts in normal and GHRKO mice.
Conclusion: Whereas expression of apoptosis-related genes is decreased in skeletal muscles, but not in hearts, of GHRKO mice, it is not clearly affected by caloric restriction in either tissue (supported by NIA).
Article tools
My recent searches
My recently viewed abstracts
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more