ECE2010 Poster Presentations Growth factors (15 abstracts)
The role of fibroblast grow factor 23 in vascular calcification
Bohumil Majtan 1, , Tatsuo Shimosawa 2 , Rika Saito 2 , Hong Wang 2 , Shengyu Mu 2 , Fumiko Mori 2 , Yuzaburo Uetake 2 , Robert Holaj 1 & Toshiro Fujita 2
13rd Medical Department, 1st Faculty of Medicine at Charles University and General Teaching Hospital in Prague, Prague, Czech Republic; 2Department of Nephrology and Endocrinology, University of Tokyo, Tokyo, Japan.
Background: Patients in end stage chronic kidney disease suffer from vascular calcification have high plasma level of phosphate
The aim of our work was to investigate the role of FGF23 in vascular calcification.
Methods: Aortic tissue and primary culture of vascular smooth muscle cells (VSMC) both obtained from Sprague–Dawley rat were used during the experiment. Klotho plasmid was first transfected into VSMC and cultures were subsequently cultivated in high (2.6 mmol) and low (1.4 mmol) phosphate buffer solutions. Same medium was used for cultivation of aortic tissue. FGF23 was then added to cultivation medium and expression of core osteoblast-like phenotype change markers Pit-1 and Cbfa1 and involved transcription factors were examined.
Results: The expression of Pit-1 and Cbfa1 was upregulated by high
Conclusion: We successfully downregulated the key osteoblast-like phenotype change markers Pit-1 and Cbfa1, which are induced by high phosphate buffer, by FGF23. This might demonstrate the possible protective effect of FGF23 in vascular calcification.
Volume 22
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Endocrine Abstracts
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