ECE2008 Poster Presentations Neuroendocrinology (107 abstracts)
A comparison between three automated chemiluminescence assays for growth hormone: on the use of recombinant hGH as a primary calibrant
Ayman M Arafat 1 , Matthias Möhlig 1 , Martin O Weickert 1 , Frank H Perschel 2 , Johannes Purschwitz 1 , Joachim Spranger 1 , Christian J Strasburger 3 , Christof Schöfl 4 & Andreas FH Pfeiffer 1
1Department of Endocrinology, Diabetes and Nutrition, Charité-University Medicine Berlin, Campus Benjamin Franklin, Berlin, Germany; 2Department of Clinical Chemistry and Pathobiochemistry, Charité-University Medicine Berlin, Campus Benjamin Franklin, Berlin, Germany; 3Department of Clinical Endocrinology, Charité-University Medicine Berlin, Campus Mitte, Berlin, Germany; 4Division of Neuroendocrinology, Department of Neurosurgery, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Objectives: GH measurements during OGTT have profound effects on therapy and follow-up management of acromegaly. To minimize the discordance between immunoassays, it is recommended to calibrate them using 22 kDa-GH-preparation instead of the use of pituitary-derived calibrants. The aim of our study was to evaluate the between-method discrepancies in GH determinations by assays using different calibrants considering further confounders like age, gender, and BMI.
Methods: GH was measured during a 75-g OGTT in 21 acromegaly patients (9 controlled; 6 men; age 34–63 years; BMI 25.8±0.7 kg/m2) and in 207 apparently healthy subjects (64 men; age 20–76; BMI 30±0.5) using three Chemiluminescence-assays. Immulite2000 and Nichols were calibrated against NIBSC2ndIS98/574 for hGH, whereas LIAISON used recombinant-hGH as a primary calibrant. The Nichols-assay was re-calibrated using rhGH-preparation.
Results: The results obtained with all assays were strongly correlated in both acromegaly patients and controls (r=0.96 and 0.99, respectively; P<0.001). The results obtained with Immulite2000 were 2-fold higher than those obtained with LIAISON and Nichols; the readings of the two latter methods were in good agreement. In controls, nadir GH concentrations were (95% percentile) 0.25, 0.41 and 0.2 for LIAISON, Immulite2000 and Nichols, respectively. Using cut-off limits of 0.5 μg/l (LIAISON) and 1 μg/l (Immulite2000) identified 92–100% of patients with active disease and 67% of patients in remission.
Both basal and nadir GH levels were significantly higher in females than in males (LIAISON: 1.3±0.17 vs. 0.46±0.1 μg/l and 0.13±0.01 vs. 0.1±0.02 μg/l, P<0.001, respectively).
In multiple regression analysis age, BMI and gender were independent predictors for both the basal and the nadir GH levels (standardized β: −0.2, −0.3 and 0.2, respectively).
Conclusions: Post-glucose GH-nadir values are assay-, gender-, age-, and BMI-specific. The use of rhGH as a primary calibrant is advisable to reduce the between-assay differences in GH measurements.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more