SFEBES2008 Oral Communications Tumours, diabetes, bone (8 abstracts)
Human omental adipose tissue as a key site for the inflammatory 15-lipoxygenase pathway: Implications for metabolic disease
Margaret J Hill , Philip G McTernan , Nancy F da Silva , Kirsty C McGee , Christine M Kusminski , Adam R Baker , Sudhesh Kumar & Alison L Harte
Warwick Medical School, Clinical Sciences Research Institute, Coventry, UK.
Materials and methods: Paired AbSc and Om AT were collected from patients undergoing elective liposuction surgery (Age; 45±1.72 years; lean BMI; 22.91±0.56 kg/m2; obese BMI; 33.51±1.04 kg/m2; n=23) for gene expression analysis by microarray and real time polymerase chain reaction (PCR). Fasted blood was taken to measure metabolic parameters.
Results: Microarray data showed that Om AT had significantly higher expression of 15-LO (P=0.00016) than paired AbSc AT, independent of BMI. In addition, other key elements of AA metabolism; prostaglandin D2 synthase (P=0.000008), leukotriene A4 hydrolase (P=0.00036), prostaglandin endoperoxide synthase 1 (P=0.000001) and cytochrome p450 (P=0.000075) showed significantly higher expression in Om AT. PCR data further corroborated the 15-LO expression profile (AbSc AT: ΔCt 17.10±0.86 and Om AT: ΔCt 6.48±0.37 (P=<0.001)) independent of BMI.
Conclusion: These studies highlight, for the first time, that 15-LO is clearly differentially expressed in human AT. Furthermore that omental AT, which is associated with increased inflammatory response, also has increased mRNA expression of the 15-LO pathway. Taken together, these findings suggest a previously undefined mechanism within human fat that may exacerbate the inflammatory response in obesity mediated T2DM.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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