Relationship between sodium iodine symporter and thyroid peroxidase expression and cytokines production by thyrocytes from patients with immune and non-immune thyroid disorders

A Bossowski; B Czarnocka; A Stasiak-Barmuta; K Bardadin; M Urban; A Lyczkowska; J Czerwinska; J Dadan

P773

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Endocrine Abstracts (2006) 11 P773

ECE2006 Poster Presentations Thyroid (174 abstracts)

Relationship between sodium iodine symporter and thyroid peroxidase expression and cytokines production by thyrocytes from patients with immune and non-immune thyroid disorders

A Bossowski ¹ , B Czarnocka ² , A Stasiak-Barmuta ³ , K Bardadin ⁴ , M Urban ¹ , A Lyczkowska ² , J Czerwinska ⁴ & J Dadan ⁵

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¹2nd Department of Children’s Diseases. Medical University, Biaystok, Poland; ²Department of Biochemistry. Medical Center of Postgraduate Education, Warsaw, Poland; ³Department of Pediatric Allergology, Bialystok, Poland; ⁴Department of Patomorphology, Medical Center of Postgraduate Education, Warsaw, Poland; ⁵2nd Dep of Children’s Diseases. Medical University, Bialystok, Poland.

The course of Graves’ disease is associated with the inflow of lymphocytes to the thyroid gland and dysregulation of the immune system characterized by reaction to thyroid antigens (peroxidase, thyroglobulin, TSH receptors and Na⁺/I⁻ symporter).

The aim of this study was to estimate sodium iodine symporter (NIS) and thyroid peroxidase (TPO) expression in thyrocytes which release cytokines INF-γ and IL-4 from young patients with Graves’ disease (GD) and no-toxic multinodular goitre (NTMG). The investigation was performed on thyroid cells isolate from postoperation thyroid tissues from 12 patients aged 11–18 years old with GD and 12 cases aged 13–18 years old with NTMG. Detection of NIS and TPO in thyrocytes performed by immunohistochemistry using mAb-47 and anti-NIS antibodies in DAB chromogene visuality and marked by Mayer’s haematoxylin. Additionally, TPO identified by Western Blot method with mAb-47. Analysis of cytokines from isolated and cultured thyrocytes was performed using antibodies to IL-4 -PE and IFN-γ-PE by flow cytometry.

The analysis of expression of NIS and TPO in thyrocytes was higher in patients with GD in compared to their detection in patients with NTMG. In addition, degree of thyroid antigen expression positive correlated with amount of cytokines production (INF- γ, P<0.05; IL-4, P<0.02). Furthermore, detection of IL-4 and INF- γ was significantly higher in thyrocytes from Graves’ patients (P<0.01; P<0.05, respectively) in comparison to the NTMG patients.

We conclude that elevated expression of NIS and TPO in Graves’ disease is associated with higher stimulation and activation of thyroid follicular cells in inflammatory process within thyroid gland.

Approved by the Local Ethical Committee.