ECE2006 Poster Presentations Thyroid (174 abstracts)
Antiproliferative effects of SOM230 on orbital fibroblasts from active Graves’ ophthalmophaty
D Pasquali , V Rossi , G Bellastella , D Esposito , MC Quinto , A Bellastella & AA Sinisi
Second University of Naples, Dip Internistica Clinica e Sperimentale, Sezione di Endocrinologia, Napoli, Italy.
Recently it has been shown that somatostatin (SST) might be of therapeutic value in the treatment of active Graves’ophthalmopathy (GO). SST and somatostatin receptors (sst) transcripts are expressed and functional in cultured orbital fibroblasts deriving from GO patients (FGO). Octreotide, the SST analogue currently used is able to inhibit in vitro growth and activity of FGO. New analogues with wider sst-affinity are now available for in vitro experimental studies. In this study we investigated the effects of the new synthetic SST analogue SOM230 on the control of growth and apoptosis in FGO.
Methods: Cells, cultured in dMEM, were starved without FCS for 2 days, then treated. with 10 nM, 100 nM SOM230 for 48 h. For the analysis of proliferation and apoptosis, cells were harvested after treatment, and analyzed by MTT and TUNEL techniques.
Results: FGO, after 48 h treatment with 100 nM SOM230, showed a significant cell growth reduction and induction of apoptosis in 15% of cells.
Conclusions: These data demonstrate that SOM230 is able to control cell growth and induce programmed cell death in FGO and might be another therapeutic tool available for active GO control.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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