Endocrine Abstracts

Objective: To study the protective effect of grape procyanidins on hydrogen peroxide induced oxidative damage in MC3T3-E1 osteoblast-like cells. Methods: Cultured MC3T3-E1 Osteoblast-like cells were treated with grape procyanidins and 125 micromole per litre hydrogen peroxide for six hours. The levels of apoptosis, mitochondrial transmembrane potential and proliferation were subsequently analyzed by flow cytometry and Bio-Red model 550 instrument. Results: The proliferation level in 50 milligram per litre GPC protective group was 0.258±0.018, significantly higher than the normal control and hydrogen peroxide group (P<0.05). The apoptotic rate and mitochondrial transmembrane potential of the positive group were 29.53±3.8 (%) and 27.24±2.2 (D value) respectively. With co-treatment of 50 milligram per litre GPC, the levels of apoptosis and mitochondrial transmembrane potential were 8.61±1.2 (%) and 87.55±4.7 (D value). The differences between the two groups were significant (P<0.05). Conclusion: Our data indicates the important effect of grape procyanidins in the prevention of bone loss by protecting against the oxidative damage of free radicals in MC3T3-E1 osteoblast-like cells, in addition to stimulating their proliferation.