BES2005 Poster Presentations Diabetes and metabolism (35 abstracts)
The effect of a high fat, high cholesterol diet on blood glucose in pigs
KA Massey 1 , J Laws 1 , KS Perkins 1 , S Beattles 1 , L Filby 1 , E Hatton 1 , MA Lomax 1 , DO Haskard 2 , J Buckingham 3 , MR Jarvelin 4 , AM Corson 1 , J Lean 1 & L Clarke 1
1DAS, Imperial College, London, UK; 2NHLI, Imperial College, London, UK; 3Department of Neuroscience and Psychological Medicine, Imperial College, London, UK; 4Department of Epidemiology Public Health, Imperial College, London, UK.
Epidemiological studies have suggested that in man low birth weight followed by rapid catch-up growth dramatically increase the risk of adulthood diabetes and metabolic syndrome. This experiment investigated the effect of a high fat, high cholesterol post-weaning diet on blood glucose levels of low (L) and normal (N) birth weight pigs.
Low (L: <10thpercentile; n=6) and normal (N: 40th - 60th percentile; n=12) birth weight piglets were selected. Pigs were randomly allocated to either a standard diet (CON: L, n=3; N, n=6) or the standard diet plus 15% (w/w) palm oil and 2%(w/w) cholesterol (FAT: n=3; N n=6) from 3 months of age for a 60-day period when a glucose tolerance test (GTT) was performed by administering glucose (0.5 g/kg live weight, i.v.). Blood glucose concentration was assessed using an Esprit 2 glucometer (Bayer, UK). Data was analysed using General Linear Model, ANOVA; values are presented as mean±standard deviation.
Basal glucose was similar in CON and FAT L pigs (4.06±0.41 mM) and was higher (P<0.01) in the N pigs offered the FAT diet (CON, 3.64±0.71: FAT 4.41±0.6 mM). At 30 mins following the GTT, glucose concentration was lower (p<0.05) than basal levels in CON L pigs (L, 2.81±0.98; N, 3.52±0.96 mM) but all other glucose values had returned to basal values.
The basal glucose levels support the hypothesis that pigs fed a high fat, high cholesterol diet are insulin resistant and that low birth weight is associated with insulin resistance in the control fed pigs. Blood glucose levels after the GTT suggest that FAT induced insulin resistance is maintained in CON pigs and low birth weight alters the relationship between the FAT diet and insulin resistance. The study was funded by Imperial College London.
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Endocrine Abstracts
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