BES2003 Poster Presentations Reproduction (22 abstracts)
Hypoxia influences steroid and nitric oxide production in swine granulosa cells
F Bianco 1 , F Grasselli 1 , G Basini 1 , M Tirelli 1 , S Bussolati 1 , V Cavalli 1 & C Tamanini 2
1Sezione di Fisiologia Veterinaria, Facoltà di Medicina Veterinaria, Università di Parma,Italia; 2DIMORFIPA,Sezione di Fisiologia Veterinaria, Facoltà di Medicina Veterinaria, Università di Bologna, Italia.
It is well-known that follicular development is associated with many morphological and functional changes. As long as the follicle develops and matures to the preovulatory stage, the availability of oxygen progressively diminishes and the environment becomes hypoxic; one of the most important effects induced by hypoxia on granulosa cells is the induction of angiogenesis. Our aims were: 1) to investigate whether hypoxia alters the production of estrogen and progesterone in swine granulosa cells and 2) to verify its possible effects on NO production, whose involvement in the angiogenic process has been already documented. To this purpose swine granulosa cells were obtained from large follicles (> 5mm) and cultured in M199 + 0.1% BSA in normoxic (O2 21%, controls), partial hypoxic (O2 5%) or anoxic (O2 1%) conditions. After a 18 h incubation, media were collected and assayed for estradiol 17beta (E2) and progesterone (P4) production by validated RIAs and for NO output by Griess assay. Both P4 and E2 production was reduced by 20% vs control (p<0.05) in granulosa cells subjected to anoxia, while no significant differences were observed in the partial hypoxic group. NO production was reduced by 50% (p<0.05) both in hypoxic and anoxic condition. On the basis of these data, a partial reduction of O2 (condition that likely mimicks the in vivo one) does not seem to be effective in reducing progesterone and estrogen production; on the contrary, severe hypoxic conditions inhibit steroid production. As for the nitric oxide, it is known that it plays an antiangiogenic role by inhibiting VEGF production: its reduction in hypoxic condition may suggest its involvement as a mediator of the pro-angiogenic effect of hypoxia. This work was supported by a COFIN grant.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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