SFE2001 Poster Presentations Endocrine tumours and neoplasia (10 abstracts)
Variable expression of IGF-1 in breast cancer influences expression of c-myc and Cox-2
C Laban 1 , W Ogunkolade 2 , K Kirkpatrick 1 , S Bustin 3 , K Mokbel 4 , M Ghilichik 5 , R Carpenter 1 & PJ Jenkins 2
Depts of Breast Surgery1, Endocrinology2and Academic Surgery3St Bartholomew's and The Royal London; Dept of Breast Surgery, St Georges Hospital4and Dept of Pathology, Central Middlesex Hospital5, London
Background: IGF-1 is a ubiquitous growth factor that has been shown to play an important role in breast tissue development and tumorigenesis. Previously we have shown that IGF-1 expression is lost in approximately 1/3 of breast carcinomas. It is unsure whether this indicates the existence of a subset of breast cancers that may behave differently clinically and on a molecular level. Aims: To assess and quantify the mRNA expression of (i) Cox-2 and c-myc, genes implicated in breast tumourgenesis, and (ii) GH, GH receptor, IGF-1R and IGFBP-3, in the two groups (IGF-1 positive and IGF-1 negative) of breast carcinoma. Methods: Total RNA was extracted from samples of 32 breast cancers, of which 13 had absent IGF-1 expression. Using a real-time, hydrolysis probe dependent RT-PCR assay ('Taqman'), transcription was quantified and expressed as copy number/µg total RNA. Results: Expression of both c-myc and Cox-2 was significantly higher in the IGF-1 positive tumours. In addition the expression of GHR and IGFBP-3 was also increased in the IGF-1 positive breast cancer group. There was no difference in GH or IGF-1R expression between groups.
| c-myc | Cox-2 | GH-R | IGFBP-3 | |
| IGF-1+ve carcinoma | 1.0E+08 (1.1E+09- 4.6E+07) | 2.4E+06 (4.2E+07- 6.0E+05) | 2.4E+07 (1.6E+09- 3.3E+05) | 1.2E+07 (8.0E+07- 9.5E+05) |
| IGF-1-ve carcinoma | 5.7E+07* (4.2E+08- 2.9E+06) | 9.9E+05* (2.4E+07- 8.9E+04) | 2.9E+06* (5.0E+07- 1.54E+02) | 4.5E+06* (7.6E+07- 2.24E+04) |
Conclusions: There are two distinct groups of breast carcinoma, with respect to IGF-1 expression. The reduced expression of c-myc and cox-2 in the IGF-1 negative group suggests a lower level of proliferation and angiogenesis. However the clinical significance of these differences remains to be determined.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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